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Image Search Results
Journal: PLoS ONE
Article Title: Comparison of Static and Microfluidic Protease Assays Using Modified Bioluminescence Resonance Energy Transfer Chemistry
doi: 10.1371/journal.pone.0088399
Figure Lengend Snippet: All treatments included 1 µM GFP 2 -RG-RLuc. The control was 1 µM RLuc, 1 µM GFP 2 and 5 µM native coelenterazine. Thrombin pre-treatment was 54 nM thrombin for 90 minutes at 30°C. The thrombin+Hirudin condition involved addition of 2 units of hirudin at room temperature 10 minutes before addition of 54 nM thrombin. All data were measured in a 96 well microplate using a SpectraMax M2 spectrofluorometer.
Article Snippet: Simultaneous dual emission hybrid BRET measurements were carried out in a
Techniques: